Pulsatile Dynamics in the Yeast Proteome

The activation of transcription factors in response to environmental conditions is fundamental to cellular regulation. Recent work has revealed that some transcription factors are activated in stochastic pulses of nuclear localization, rather than at a constant level, even in a constant environment. In such cases, signals control the mean activity of the transcription factor by modulating the frequency, duration, or amplitude of these pulses. Although specific pulsatile transcription factors have been identified in diverse cell types, it has remained unclear how prevalent pulsing is within the cell, how variable pulsing behaviors are between genes, and whether pulsing is specific to transcriptional regulators or is employed more broadly. To address these issues, we performed a proteome-wide movie-based screen to systematically identify localization-based pulsing behaviors in Saccharomyces cerevisiae. The screen examined all genes in a previously developed fluorescent protein fusion library of 4,159 strains in multiple media conditions. This approach revealed stochastic pulsing in ten proteins, all transcription factors. In each case, pulse dynamics were heterogeneous and unsynchronized among cells in clonal populations. Pulsing is the only dynamic localization behavior that we observed, and it tends to occur in pairs of paralogous and redundant proteins. Taken together, these results suggest that pulsatile dynamics play a pervasive role in yeast and may be similarly prevalent in other eukaryotic species

Prevalence of Cryptosporidium spp. infection in renal transplant and hemodialysis patients

Transplanted and hemodialysis patients are frequently affected by parasitic diseases such as cryptosporidiosis. Cryptosporidium is a parasite causing self-limited diarrhea and enteritis in healthy individuals. The presence of Cryptosporidium infection was studied in three groups including 87 renal transplant patients, 103 hemodialysis patients, and 60 healthy individuals as the control group. Two stool specimens were obtained from each case. The specimens were concentrated by the formalin-ether method and two smears were prepared from each. The smears were stained by modified acid-fast method and were observed under a light microscope. Ten (11.5) renal transplant and 4 (3.88) hemodialysis patients were positive for Cryptosporidium infection. No positive results were obtained in the control group. The results showed a statistically significant difference between renal transplant and control groups (P=0.02), but the difference between hemodialysis and control groups was not significant (P=0.2). The results also showed that the rate of Cryptosporidium infection in renal transplant patients was much higher than hemodialysis patients. The susceptibility of renal transplant patients to Cryptosporidium infection is much more than other studied groups and this could be due to immunosuppressive therapy in these patients

Effects of water temperature and migration time on some fecundity indices and fertilization rate of female Kutum, Rutilus frisii kutum,migratory to Shiroud River in the southwest Caspian Sea

Kutum, Rutilus frisii kutum, is an commercially important fish in the Caspian Sea. The fish enters the rivers leading to the Caspian Sea for spawning. One of these rivers is Shiroud River. In the artificial propagation process of Kutum, different factors are involved in quality and quantity of female broods eggs and also in the best temperature and migration time for spawning. The influence of temperature and migration time on some fecundity indices and fertilization rate of female kutum in Shiroud River was studied in the spawning season. In this study, 90 individual females were studied from February to May 2007. Averages of total length, weight and age were 43.26 cm, 832.08 g, and 4.41 respectively. Results showed that maximum egg diameter (1.86 mm), number of eggs per each gram of body weight (309.12), relative fecundity (56.21) and fertilization rate (95.82 %) were obtained from 5 to 20 April (15.95°C). Maximum ovary weight (201.00 g) and absolute fecundity of eggs (49987.18) were obtained from 6 to 20 March (17.74°C). Relationship between temperature and migration time and fecundity indices was linear and weak

Synovial cavernous hemangioma with juxta-articular hemangioma in a 29-year old woman: A case report

Introduction: Synovial hemangiomas are a rare benign vascular malformation that most commonly affects the knee joint, usually involving the anterior compartment. Histopathology examination is considered the gold standard for the diagnosis of synovial hemangioma. Surgical excision, either done per arthroscopy or per arthrotomy, is the treatment of choice. Presentation of case: This study presents a 29-year-old female admitted to our hospital in March 2020 who complained of continuous pain, swelling, and recurrent haemarthroses without a history of trauma for six months. The anteroposterior and lateral plain radiographs of the left knee showed no abnormality. An ultrasound of the left knee showed lobulated hypoechoic lesions in intra-articular and infra-suprapatellar pouches. Multiple vessels within the lesion with the low-velocity venous flow have appeared in color-Doppler imaging. Magnetic resonance imaging (MRI) of the left knee showed an irregular soft tissue mass in intra-articular fossa that infiltrating infra-suprapatellar pouches along to vastus medialis muscle (juxta-articular areas) measuring about 87 � 72 � 75 mm. Synovial effusion and bone erosions were notable. Fine-needle aspiration cytology (FNAC) offered hemarthrosis. The excisional biopsy obtained from the lesion and imprint cytology performed immediately after tissue removal. The cytologic diagnosis was compatible with a benign vascular neoplasm. The histologic exam confirmed synovial hemangioma. Discussion: Synovial hemangioma is a rare benign tumor of vascular origin arising from synovium-lined tissues, and often affects adolescents and young adults. Synovial hemangioma is often associated with an adjacent cutaneous or deep soft tissue hemangioma. It is a vascular malformation that contains variable amounts of adipose, fibrous, and muscle tissue, as well as thrombi in the vessels. At present, MRI has become the modality of choice for the evaluation of hemangiomas. The final diagnosis established with the histologic examination. The choice treatment is surgical excision. Conclusion: Although synovial hemangioma is a rare condition, be considered for non-specific clinical symptoms. However, an early diagnosis of synovial hemangioma is fundamental for adequate treatment. © 2020 The Author

A simplified model for calculating atmospheric radionuclide transport and early health effects from nuclear reactor accidents

During certain hypothetical severe accidents in a nuclear power plant, radionuclides could be released to the environment as a plume. Prediction of the atmospheric dispersion and transport of these radionuclides is important for assessment of the risk to the public from such accidents. A simplified PC-based model was developed that predicts time-integrated air concentration of each radionuclide at any location from release as a function of time integrated source strength using the Gaussian plume model. The solution procedure involves direct analytic integration of air concentration equations over time and position, using simplified meteorology. The formulation allows for dry and wet deposition, radioactive decay and daughter buildup, reactor building wake effects, the inversion lid effect, plume rise due to buoyancy or momentum, release duration, and grass height. Based on air and ground concentrations of the radionuclides, the early dose to an individual is calculated via cloudshine, groundshine, and inhalation. The model also calculates early health effects based on the doses. This paper presents aspects of the model that would be of interest to the prediction of environmental flows and their public consequences

Intraoperative 3-D mapping of parathyroid adenoma using freehand SPECT

Background: Freehand single photon emission computed tomography (fSPECT) is a three-dimensional (3-D) tomographic imaging modality based on data acquisition with a handheld detector that is moved freely, in contrast to conventional, gantry-mounted gamma camera systems. In this pilot study, we evaluated the feasibility of fSPECT for intraoperative 3-D mapping in patients with parathyroid adenomas. Methods: Three patients (range 30 to 45 years) diagnosed with hyperparathyroidism (one primary and two tertiary) underwent parathyroid scintigraphy with technetium-99m sestamibi (99mTc-MIBI) to localize parathyroid adenomas. Two patients were referred with persistent hyperparathyroidism after conventional parathyroidectomy. In all three patients, a planar scintigraphy of the neck was performed 10 min after injection (p.i.) followed by SPECT/CT (Symbia T2, Siemens Healthcare) and a correlative ultrasound 2 h p.i. 99mTc-MIBI scan was performed the day before surgery in two patients and at the same day in one patient. fSPECT images were acquired intraoperatively using declipse SPECT (SurgicEyeTM). Results: A total of five parathyroid adenomas were successfully located with SPECT/CT. fSPECT allowed intraoperative detection of all adenomas, and successful parathyroidectomy was accomplished. Parathyroid hormone level decreased intraoperatively in all three patients, on average, by 79% (range 72% to 91%). Conclusion: In this preliminary study, we could demonstrate that intraoperative localization of parathyroid adenomas is feasible using the freehand SPECT technology, thus allowing an image-guided parathyroidectomy

Optimizing psma radioligand therapy for patients with metastatic castration-resistant prostate cancer. A systematic review and meta-analysis

The aim of the review was to evaluate patient and treatment characteristics for patients with metastatic castration-resistant prostate cancer (mCRPC) treated with PSMA radioligand therapy (PRLT) associated with above-average outcome. The systematic review and meta-analysis followed recommendations by the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA). We searched for publications in PubMed, Embase, and ClinicalTrials.gov up to 31 September 2020. Thirty-six publications and four duplicates reported 2346 patients. Nearly two-thirds of the patients had bone metastases. Median overall survival (OS) was 16 months. Asymptomatic patients and patients with only lymph node metastases lived longer than symptomatic patients and patients with more extensive metastases. Patients treated with an intensified schedule of177Lu PRLT lived longer than those treated with a conventional schedule. Half of the patients obtained a PSA decline ≥ 50% and these patients lived longer than those with less PSA decline. Approximately 10% of the patients developed hematologic toxicity with anemia grade 3 as the most severe adverse effect. Characteristics for patients, cancer, restaging, and PRLT predict above average overall survival following treatment with PRLT